In order to reduce particulate matters (PM) from marine diesel engines, we developed novel electrostatic diesel particulate matter filtration system. Electrostatic diesel particulate filtration (DPF) system consists of electrostatic charger and filtration part. The electrostatic charger and filtration part are composed of a metal discharge electrode and a metal fiber filter (porosity: 68.1-86.1%), respectively. In the electrostatic charger part, diesel soot particles are reduced by electrostatic force. The filtration part after electrostatic charger part reduces diesel soot particles through inertial and diffusion forces. The filtration efficiency of electrostatic DPF system was examined through the experiments using engine dynamometer system (300 kW) and ship (200 kW). The PM reduction efficiencies due to inertial and electrostatic forces showed about 70-75% and 80-90%, respectively, according to the RPM of the engine. The differential pressure of the electrostatic DPF system applied to the ship was about 1-9 mbar, which was less than the allowable differential pressure for ship engines in South Korea (100 mbar). The results show that the electrostatic DPF system is suitable for application to the PM reduction emitted from ships.