Currently, droplet protective screens (DPSs) are used to prevent the spread of respiratory diseases. As virus particles can maintain their infective in indoor environments, recent studies have investigated the risk of airborne transmission. However, the ability of DPSs to block airborne transmission has not been verified yet. In this study, the preventive ability of DPSs against droplet and airborne transmission was evaluated. Moreover, the effectiveness of a Portable air purifier (PAP) was investigated. According to results, in a simulated room where an infectious person spoke, the DPS blocked more than 90% of the micron-sized droplets (with a diameter larger than 1 µm) transmitted to the front of the infectious person. However, sub-micron droplets (with a diameter smaller than 1 µm) passed through the DPS and spread in a room. However, the PAP reduced the amount of both micron and sub-micron droplets transmitted to the front of the infectious person. When the PAP airflow direction was set from the DPS surface to the free space near the infectious person, improved prevention against droplet and airborne transmission was recorded. However, airborne transmission was accelerated when the PAP　airflow direction was set from the free space to the DPS surface.