A new depth filter media was designed and samples of flat sheet as well as cartridge assembly were prepared and tested to evaluate the filtration performance and compare with the commercial product. The arrangement of the depth filter media layers is important to reach the optimal filtration parameters like filter pressure drop, particle collection efficiency and dust holding capacity. Initially, both flat sheet samples of new media and commercial product have been tested using standard test units. Tests with new depth filter media cartridges of various pleat count were conducted in order to find the optimal pleat count which would represent the lowest pressure drop. These tests give an insight on how the pleat count and the assembly configuration affect the performance of the depth filter media cartridge. By comparing the samples with a commercial product we could confirm relatively high filtration performance of the sample cartridge with pleat count 150 made of new depth filter media. The cartridge with the same pleat count exhibits better performance without the outer mesh. Keywords：Depth filter media, Depth filter cartridge, Pressure drop, Particle collection efficiency, Pleat count