Particle and Aerosol Research Vol. 12, No. 1, March 2016, Pages 1-9 |
ISSN : 1738-8716 (Print) ISSN : 2287-8130 (Online) |
Characteristics of flow field of nose-only exposure chamber for inhalation toxicity evaluation
Hakjae Noh1), Choonkeun Bong1), Hakyung Bong1), Yonggu Kim1)*, Myunghaing Cho2), Sanghwa Kim2), and Daesung Kim3)
1)Greensolus Co., Ltd., Seoul, Korea
2)Laboratory of Toxicology, Research Institute for Veterinary Science and College of Veterinary Medicine, Seoul National University, Seoul, Korea
3)Global Frontier Center for Multiscale Energy Systems, Seoul National University, Seoul, Korea
*Corresponding author. Tel.: +82-(0)70-8290-5060, E-mail: greensolus@greensolus.co.kr
Received 30 October 2015, Revised 11 January 2016, Accepted 12 February 2016, Available online 31 March 2016
http://dx.doi.org/10.11629/jpaar.2016.12.1.001
Abstracts
The test was done with these 3 types of chamber with different form to develop inhalation toxicity evaluation system, easy-to-operate system among exposure chamber used for evaluating inhalation toxicity of environmental chemical mixtures. Through CFD interpretation, nose-only exposure chamber was made with the selection of the optimal conditions. For its evaluation, one type of fragrance was selected and measured particle size distribution of each port. The gene becoming luminous to green fluorescence was combined with GPT-SPE, a type of tGFP vector, to be inhaled to the mouse. Based on this, luminous intensity was checked. As a result, total particle number concentration of each port had average value of 3.17¡¿106 #/cm3 and range of the highest and lowest concentration value was approximately ¡¾4.8%. Autopsy of lung tissues of mouse showed that it had clearly better delivery of gene compared to the control group.
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